SPRY4 抗体-抗体-抗体-生物在线
SPRY4 抗体

SPRY4 抗体

商家询价

产品名称： SPRY4 抗体

英文名称： SPRY4 Antibody

产品编号： XY22765-1-

产品价格： null

产品产地： 中国/美国

品牌商标： XYbscience

更新时间： 2023-08-17T09:55:27

使用范围： WB,ELISA,IHC-P,IHC-F,IF

上海信裕生物科技有限公司
  • 联系人 : 徐经理
  • 地址 : 上海市闵行莘庄工业区春东路508号A1-2F
  • 邮编 : 200612
  • 所在区域 : 上海
  • 电话 : 152****8802
  • 传真 : 021-37680378
  • 邮箱 : shxysw02@163.com
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 SPRY4 抗体Applications

In-house applications ELISA, WB, IHC, FC     Application not tested? Please see our Guarantee.
Species specificity Human; other species not tested.
Images
Prev1NextView more validation data
  • 22765-1-AP;A375 cellsA375 cells were subjected to SDS PAGE followed by western blot with 22765-1-AP( SPRY4 Antibody) at dilution of 1:1000
  • 22765-1-AP;human cervical cancer tissueImmunohistochemistry of paraffin-embedded human cervical cancer tissue slide using 22765-1-AP( SPRY4 Antibody) at dilution of 1:50 (under 40x lens)
  • 22765-1-AP;HeLa cells1X10^6 HeLa cells were stained with 0.2ug SPRY4 antibody (22765-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
Positive WB detected in A375 cells
Positive IHC detected in Human cervical cancer tissue, human heart tissue
Positive FC detected in HeLa cells
Recommended dilution WB : 1:500-1:5000
IHC : 1:20-1:200
 SPRY4 抗体Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
Product Information
Source Rabbit Purification method Antigen Affinity purified
Isotype IgG Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC.
Immunogen Information SPRY4 抗体
Immunogen SPRY4 fusion protein ag18574 Full name Sprouty homolog 4 (Drosophila)
Calculated molecular
weight		 299aa,33 kDa Observed molecular 
weight		 35-38 kDa
GenBank accession 
number		 BC125095 Gene ID (NCBI) 81848
Gene symbol SPRY4 Synonyms Protein sprouty homolog 4, sprouty homolog 4 (Drosophila), Spry 4, SPRY4
Background

SPRY4, also named as Protein sprouty homolog 4, is a 299 amino acid protein, which contains one SPR (sprouty) domain and belongs to the sprouty family. SPRY4 Suppresses the insulin receptor and EGFR-transduced MAPK signaling pathway, but does not inhibit MAPK activation by a constitutively active mutant Ras. Probably impairs the formation of GTP-Ras. SPRY4 has a cellular function to regulate the actin cytoskeleton, independent of its inhibitory activity on the Ras/MAP kinase signalling. SPRY1, SPRY2, SPRY3, SPRY4, are central and complex regulators of the receptor tyrosine kinase (RTK) signalling pathway. SPRY4 抗体

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